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Simmonds P , Davidson F , Jarvis LM . 1998. Transfusion transmitted virus - Reply LANCET , 352 (9136), pp. 1310-1311. | Read more
Haydon GH , Jarvis LM , Simmonds P , Hayes PC . 1998. Hepatitis G virus in patients with hepatocellular carcinoma. HEPATOLOGY , 28 (4), pp. 219A-219A.

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Simmonds P . 1998. Transfusion virology: progress and challenges. Blood Rev , 12 (3), pp. 171-177. | Show Abstract | Clearance Outlet Locations Giuseppe Zanotti Espadrilles Gipsy suede crystals ornament bast Cheap Finishline iPqHQL

Discoveries of new human viruses and new technologies for their detection have made, and will continue to make, major contributions to the safety of blood transfusion. This article discusses the practical issues involved in the implementation of additional serological screening tests for viruses such as human T-lymphotropic virus, and reviews current information on the prevalence and pathogenicity of more recently discovered viruses, such as hepatitis G virus (HGV) or GB virus-C (GBV-C) and human herpes virus 8, a potential aetiological agent of Kaposi's sarcoma. Progress in the technology behind nucleic acid amplification techniques, such as the polymerase chain reaction (PCR), makes direct detection of viruses such as human immunodeficiency virus and hepatitis C virus possible. The use of such methods for screening will allow the earlier detection of acutely-infected individuals and the elimination of transmission from 'window' period donations before seroconversion for antibody. Establishing a framework for PCR-based screening would also enable the testing for others such as hepatitis A virus, parvovirus B19 and GBV-C/HGV for which serological detection methods cannot be or have not been developed.

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AIMS: To evaluate the sensitivity of the Roche Cobas, Roche Amplicor plate kit, ligase chain reaction (LCR), and an in house polymerase chain reaction (PCR) by titration of purified elementary bodies (EB) and also to test 245 urethral and endocervical specimens for Chlamydia trachomatis by the four assays as well as conventional culture. STUDY DESIGN: EB titrations were run in duplicate in each commercial assay and six times in the in house PCR. Clinical samples were aliquoted and tested by each assay and were considered positive if C trachomatis was detected by two or more separate tests or if the sample was either culture or immunofluorescence positive. Major outer membrane protein (MOMP) specific primers were used as a confirmatory assay for the in house PCR. RESULTS: The in house PCR, Roche Cobas Amplicor, LCR, and Amplicor plate kit gave detection limits of approximately 1, 1-2, 2, and 2-4 EBs respectively. By the criteria described above for definition of a C trachomatis positive result in clinical samples we identified 23 true positives among the 245 clinical specimens. The in house PCR detected all 23 giving a sensitivity of 100% and a specificity of 98%. The Roche Cobas Amplicor, Roche Amplicor plate kit, and LCR detected 21, 19, and 19 of these respectively giving sensitivities of 87.5%, 82%, and 82% respectively and specificities of 99.5%, 99%, and 100% respectively. The culture gave a sensitivity of 78% and specificity of 100%. CONCLUSION: All four amplification assays had a greater sensitivity than the culture used routinely in this laboratory. The in house plasmid PCR had the greatest sensitivity and when combined with confirmation by immunofluorescence detected the greatest number of positives. This increased sensitivity is likely to have been achieved by the use of a DNA purification step and of nested primers in the amplification stage and their combined use in routine diagnostic assays for chlamydia might increase the frequency of C trachomatis detections. However, this assay is much less user friendly than the two semiautomated commercial assays investigated in this study.

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In 2012, General Motors and Verizon entered into arrangements with Prudential Insurance Company to pay the pensions of certain groups of their salaried retirees. Both companies transferred pension assets to Prudential, which meant that the GIANNI RENZI Rainbow rockstud sandals Latest KLSWOV2q
, the federal agency that insures most private pension plans, no longer insured the pensions. Since then, a number of other employers have followed suit, sparking questions and concerns from retirees who are affected.

Q: What happens to my pension when it gets transferred to an insurance company? A: If you are already getting your pension and your former employer decides to convert it to an annuity paid by an insurance company, your monthly benefit should stay the same. However, your benefits Sjp Womens Anahita ClosedToe Pumps Free Shipping Official Site Recommend Cheap Price Ebay Online MFd0KI6tw
by the federal pension insurance program, the Pension Benefit Guaranty Corporation. Instead, insurance annuities are covered by State Guaranty Associations (see below), which provide some protection in the event that insurance companies fail.If you haven’t retired yet, and your pension is transferred to an insurance company, you should make sure that both your employer and the insurance company have all of the correct information that goes into calculating your benefit. This includes dates of employment, salary history, and any survivors benefit you and your spouse have chosen. You should also make sure that you have the most recent copy of your individual benefit statement . It is not unusual for an insurance company to recalculate benefits when taking over a plan, and having this information will help to prevent disputes over how much you are supposed to receive once you start receiving your annuity. Q: What are State Guaranty Associations? A: Every state, the District of Columbia, and Puerto Rico, has a State Guaranty Association, a nonprofit institution established to protect insurance policyholders who live in that state in the event that an insurance company becomes insolvent (See this from the National Organization of Life and Health Guaranty Associations [NOLHGA]). All insurance companies that are licensed in a state are required by law to participate in that state’s State Guaranty Association. Q: What are the guarantee limits for an annuity from an insurance company? A: Guarantee limits vary by state. Each state provides a guarantee of at least $100,000 for the present value of an annuity. Tom Tailor 1694201 womens High Boots in 2018 Cheap Price Wide Range Of Sale Online Outlet Great Deals ItfTp
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to find your state’s guarantee limits. If the value of your benefit exceeds the amount protected by your State Guaranty Association, you can submit a claim for the excess in insolvency proceedings against the estate of the liquidated company. Q: How are the State Guaranty Associations paid for? A: If an insurance company is declared insolvent, the other insurance companies licensed in the state will be required to pay into the State Guaranty Association. Money in the Association and assets from the liquidation of the insolvent company are used to provide as much of the annuity benefits promised by the insolvent insurer as possible. ( See this NOLHGA FAQ ). Q: What are the requirements for receiving funds from a Guaranty Association if the insurance company providing my annuity fails? A: If an insurance company licensed in your state becomes insolvent, a State Guaranty Association will cover your individual annuity (or individual certificate under a group annuity), and those of other residents of the state, up to the Association’s limits. Individual and group annuities are the policies that insurance companies typically issue when they take over pension plans.If the insurance company is not licensed in your state, the State Guaranty Association in your state will not be responsible if the insurance company becomes insolvent. Instead, policyholders will most likely be covered by the State Guaranty Association in the state where the insurance company is incorporated. Q. How can I find out if an insurance company is licensed in my state? A: Contact your state insurance department to find out if the insurance company paying your annuity is licensed to operate in your state. The website for every state insurance commission is listed . Many of these sites allow you to search for authorized/licensed companies. Q: How often does an insurance company become insolvent? A: According to NOLHGA, since 1983 there have been approximately 100 failures of life and health insurance companies that offer policies to residents of more than one state. Most of these failures occurred during the 1990s, and relatively few have occurred recently. Q: Is there a way to assess the financial health of an insurance company? A: Each insurance company is required to file an annual report, which should be posted on its website. You can also look at the financial strength grade provided Weiss Ratings. These ratings are independently compiled and you can search for your companies’ rating via TheStreet.com . You can also pay for a detailed report from Weiss. There are other rating companies, such as A.M. Best, SP, Moody’s, and Fitch. However, they accept money from the insurance industry, which could create conflicts of interest. Q: How can I get more information about my State Guaranty Association? A: The NOLHGA website links to each of the Hogan Sneakers R260 leather metallic finished Sale Popular tV7aWnj7O
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Citation information in Europe Pubmed Central

The accuracy of different methods for the quantitation of hepatitis C virus in plasma was measured with samples from individuals infected with different genotypes and by using RNA transcripts of predetermined concentrations. Highly reproducible results were observed upon repeat testing of samples by both the original version of the Chiron branched-DNA (bDNA) assay (Quantiplex RNA assay; bDNA-1) and the currently available version (Quantiplex HCV RNA 2.0 assay; bDNA-2). A greater variability was observed in the Roche Monitor assay (correlation coefficient of 0.537, compared with 0.942 and 0.964 for the bDNA-1 and bDNA-2 assays, respectively). Significant differences in the efficiency of detection of genotypes 1, 2, and 3 were observed for the bDNA-1 and Roche Monitor assays, whereas the bDNA-2 assay and nested PCR at limiting dilution were able to quantify genotypes with equal sensitivity. By quantifying RNA transcripts of different genotypes, the sensitivities of the Roche Monitor assay for sequences of the type 2 and type 3 transcripts were estimated to be 11 and 8% of those achieved for genotype 1. When correction factors based upon these results and those from quantitation of circulating viral RNA sequences in samples from blood donors were used, the genotype-specific differences in virus load in samples from blood donors were no longer observed, consistent with previous studies with corrected values from the bDNA-1 assay. These results suggest that many of the previous studies evaluating the effect of genotype and virus load on the response to interferon using methods such as the Roche Monitor assay and other competitive PCR methods require reinterpretation. Differences in efficiency of quantitation should be taken into account in future investigations of the relationship between genotype and virus load.

Citation information in Europe Pubmed Central

BACKGROUND: Hepatitis G virus (HGV or GBV-C) is a newly discovered human flavivirus distantly related to hepatitis C virus (HCV). Little information is available on its natural history or routes of transmission, although it can be transmitted parenterally. We investigated the prevalence of persistent infection of HGV and HCV in patients exposed to non-virus-inactivated pooled blood products associated with transmission of HCV. METHODS: RNA was extracted from the plasma of 112 patients with haemophilia and 57 with hypogammaglobulinaemia, as well as from 64 different batches of archived coagulation-factor concentrates and immunoglobulins. RNA was reverse transcribed and amplified with primers from the 5' non-coding region of HCV and HGV by a nested polymerase chain reaction (PCR). Viral RNA was quantified by titration of complementary DNA before amplification. FINDINGS: Among non-renumerated UK blood donors HGV infection (detected by PCR) was more common than HCV infection (four [3.2%] of 125 compared with 137 [0.076%] of 180658 in southeast Scotland). Testing of batches of factor VIII and factor IX concentrates prepared without viral inactivation procedures showed high frequencies of contamination with HGV (16 of 17 factor VIII batches positive; six of six factor IX batches positive), with no difference between renumerated and non-renumerated donors. However, among 95 haemophiliacs who had received non-virus-inactivated concentrates, 13 (14%) were positive for HGV compared with 79 (83%) who were positive for HCV. Two of 37 recipients of long-term immunoglobulin replacement therapy were positive for HGV. Virus inactivation of blood products substantially reduced or eliminated contamination by HGV RNA sequences. INTERPRETATION: Despite the extremely high level of HGV contamination of non-virus-inactivated blood products, their use was not associated with high rates of persistent infection in recipients. The infectivity of HGV in blood products may be lower than that of HCV, or the virus may be less able to establish persistent infection in humans. Whatever the case, the high prevalence of active HGV infection in the general population remains difficult to explain.

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